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Up- or are visualized by autoradiography, in comparison to ten% enter (Input). CB: Coomassie Blue staining of RAR derivatives adsorbed on glutathione-sepharose beads. (B) Coimmunoprecipitation of AF9 and RARa in HeLa cells. HeLa cells were cotransfected with expression vectors coding for possibly wtRARa (wt) or N-terminally truncated RAR (DAF-one) with each other with an vacant pCMV-36FLAG plasmid
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Lentiviral vector carrying shRNA or total-size Vpr insert was well prepared making use of a 3-plasmid transfection technique. Briefly, the pMD.G that contains vesicular stomatitis virus glycoprotein, and pCMV-D 8.91carrying HIV-based mostly packaging plasmid were cotransfected with lentiviral vector (handle) or with the shRNA-, or Vpr-expressing lentiviral vector into 293T cells. The medium was mo
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Don't take the word of a minor on whether or not a game is appropriate for them. If a game is rated M f
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